SUITABILITY OF ROUTINE SAMPLE CONTAINERS, SAMPLING CONDITIONS, AND DIET TYPES CONDURUM ON SOME LIVER FUNCTION PARAMETERS
DOI:
https://doi.org/10.61841/cet6gz79Keywords:
Liver function tests, anticoagulants, fasting, random, starvationAbstract
Liver function tests (LFTs) are a critical investigation used to evaluate the liver's status. The accuracy of the investigation could be affected by pre-analytical factors, including sample containers, sampling conditions, and dietary types. This study is therefore designed to address the many misconceptions and disagreements associated with the above itemized preanalytical factors. A total of 150 participants were recruited for this study, and were categorized into fasting, starvation, and random. The samples were collected into plain containers, lithium heparin, K2EDTA, and fluoride oxalate containers simultaneously and separated into plasma and serum. Liver function parameters were analysed using Randox (UK) reagents on an automated chemistry analyzer, and the data on SPSS version 18, using One-Way Anova (Post Hoc-LSD). The study revealed a significant decrease (p<0.05) in the concentration of globulin in the starvation group, when compared to the control and random groups, using fluoride oxalate anticoagulant. Random samples tend to be significantly increased in the activities of the AST, ALT, and ALP when compared to the fasting and starvation groups in the plain container. Fasting, starvation, and random samples exhibited a significant decrease in concentration of globulin in K2EDTA, whereas it increased in fluoride oxalate, when compared to plain and lithium heparin containers. Furthermore, the lipid group exhibited a significant increase in ALP and AST activities when compared to the carbohydrate and protein groups, respectively. The findings of the study have shown the suitability of plain and lithium heparin containers and the preference of random or fasting sampling in LFT estimation.
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